منابع مشابه
Carboxyl-Terminal Truncations Alter the Activity of the Human α-Galactosidase A
Fabry disease is an X-linked inborn error of glycolipid metabolism caused by deficiency of the human lysosomal enzyme, α-galactosidase A (αGal), leading to strokes, myocardial infarctions, and terminal renal failure, often leading to death in the fourth or fifth decade of life. The enzyme is responsible for the hydrolysis of terminal α-galactoside linkages in various glycolipids. Enzyme replace...
متن کاملRelease of β-galactosidase from poloxamine/α-cyclodextrin hydrogels
All mammals lose their ability to produce lactase (β-galactosidase), the enzyme that cleaves lactose into galactose and glucose, after weaning. The prevalence of lactase deficiency (LD) spans from 2 to 15% among northern Europeans, to nearly 100% among Asians. Following lactose consumption, people with LD often experience gastrointestinal symptoms such as abdominal pain, bowel distension, cramp...
متن کاملα-Galactosidase-A Loaded-Nanoliposomes with Enhanced Enzymatic Activity and Intracellular Penetration.
Lysosomal storage disorders (LSD) are caused by lysosomal dysfunction usually as a consequence of deficiency of a single enzyme required for the metabolism of macromolecules, such as lipids, glycoproteins, and mucopolysaccharides. For instance, the lack of α-galactosidase A (GLA) activity in Fabry disease patients causes the accumulation of glycosphingolipids in the vasculature leading to multi...
متن کاملEffects of Different Fixatives on -Galactosidase Activity
Galactosidase ( -Gal) staining is widely used to demonstrate specific gene expression during evaluation of gene targets in vivo. This technique is extremely sensitive to fixation. Optimal fixation conditions are necessary to obtain the maximal -Gal activity. In this experiment, Carnoy’s and three different aldehyde fixatives were used at different temperatures and over different time points. Ki...
متن کاملInfluence of Bile on B-galactosidase Activity of Lactobacillus Bulgaricus
In the presence of 0.15% oxgall, the B-galactosidase activity of whole cells of Lactobacillus bulgaricus was increased. However, higher concentrations of oxgall decreased the enzyme activity. In the absence of oxgall, little or no B-galactosidase was exhibited by the whole cells. When the cells were lysed to obtain cell-free extracts prior to assay, there was some decrease in activity of the en...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1973
ISSN: 0003-6919
DOI: 10.1128/aem.26.5.783-788.1973